Regulatory

Part:BBa_K2368005

Designed by: BIT-China 2017   Group: iGEM17_BIT-China   (2017-10-01)
Revision as of 11:38, 20 October 2017 by Bitlichun (Talk | contribs)


Introduction

Pfus

Pfus is an induced promoter from Saccharomyces cerevisiae. In the endogenous MAPK pathway in S.cerevisiae,oblique.

when STE2 binding with α pheromone, STE12 will stimulate FUS1 promoter, thereby initiating the mating of yeast.

However, the sequence of Pfus contains Pst1 restrict enzyme site, so we use the method of one-step mutation to achieve the purpose of site-directed mutagenesis.(from CTGCAG to CTACAT)

T_BIT-China_2017part_K2368005.jpeg

fig.1 The sequencing result of Pfus

This part is an improvement of BBa_K775004 of 2013 SCUT.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None