Coding

Part:BBa_K2287027

Designed by: Jiang Wu   Group: iGEM17_UCAS   (2017-10-20)
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purF K326Q

It is a coding sequence of glutamine phosphoribosylpyrophosphate amidotransferase that catalyzes the transformation from PRPP to PRA in de novo purine biosynthesis. We did a mutation K326Q in this sequence based on "Identification of Sites for Feedback Regulation of Glutamine 5-Phosphoribosylpyrophosphate Amidotransferase by Nucleotides and Relationship to Residues Important for Catalysis"(Gaochao Zhou et al. ,1993) to remit the inhibition by GMP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 876
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 94
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None