Coding

Part:BBa_K2201027

Designed by: Christopher M. Whitford   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-20)
Revision as of 10:17, 20 October 2017 by Registry (Talk | contribs)

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PtNTT2(31-575)-lacO-Cas9 construct

lacO_tight2 from E. coli BL21(DE3) and optimized

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3269
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3269
    Illegal NheI site found at 3028
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3269
    Illegal BamHI site found at 5307
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3269
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3269
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None