Coding
Part:BBa_K2201027
Designed by: Christopher M. Whitford Group: iGEM17_Bielefeld-CeBiTec (2017-10-20)
PtNTT2(31-575)-lacO-Cas9 construct
lacO_tight2 from E. coli BL21(DE3) and optimized
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3269
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3269
Illegal NheI site found at 3028 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3269
Illegal BamHI site found at 5307 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3269
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3269
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |