Generator
Part:BBa_K2201242
Designed by: Svenja Vinke Group: iGEM17_Bielefeld-CeBiTec (2017-10-20)
CrtEBI under constitutive promoter with an amber codon at amino acid position 353 of crtI
This parts contains the part BBa_K274110 with an amber codon at position 353 of the crtI enzyme. With the help of an photoinducable amino acid crtI can be regulated by irradiation with light.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2037
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1573
Illegal NgoMIV site found at 1703
Illegal AgeI site found at 788 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |