Composite

Part:BBa_K2250006

Designed by: Shuailin Li   Group: iGEM17_Tsinghua-A   (2017-10-19)
Revision as of 04:33, 20 October 2017 by Shuailin10 (Talk | contribs)

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Pconst-RhlI-Pconst-RFP

This is the secretion part of warrior 1 in our project. RhlI makes the warrior 1 able to secrete the C4HSL to the medium and then enter the other cell and kill warrior 2/beggar 2/farmer 2. This can be realized by C4HSL binding to RhlR, activating the expression of LacI, and then LacI inhibiting the expression of CmR, making the warrior 2/beggar 2/farmer 2 unable to survive under the Chloramphenicol. Also, the RFP is used to distinguish between group 1 (containing warrior 1/beggar 1/farmer 1) and group2 (containing warrior 2/beggar 2/farmer 2). RFP represents group 1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 880
    Illegal NheI site found at 903
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 722
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1489
    Illegal AgeI site found at 1601
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None