![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K2230022
Designed by: Yi-Lun Huang & Pei-Hong Chen Group: iGEM17_Mingdao (2017-10-19)
STM1128/pSB1C3
Salmonella typhimurium LT2 has two glucose-specific transporter systems, PTS system and sodium/glucose cotransporter. PTS system contains two subunits IIA encoded by crr and IIBC by ptsG which are assembled to a high-affinity active transporter. The other is a Na+/glucose cotransporter encoded by STM1128 that contributes to facilitated transport with lower glucose affinity. Based on our research, the glucose transporter of Salmonella has a lower Km compared to human small intestine, Staphylococcus and E. coli, indicating a higher efficiency for glucose uptake. In our project, we created the glucose transporter device and genetically engineer microbes with these two systems.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1255
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//awards/basic_part/winner
//chassis/prokaryote/ecoli
//chassis/prokaryote/lactococcus
//collections/probiotics/production
//chassis/prokaryote/ecoli
//chassis/prokaryote/lactococcus
//collections/probiotics/production
None |