Protein_Domain
L-FABP

Part:BBa_K2302004:Design

Designed by: Hongyang Wei   Group: iGEM17_NEFU_China   (2017-10-19)
Revision as of 02:52, 20 October 2017 by Weihongyang (Talk | contribs) (→‎References)

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L-FABP,a protein that can bind long chain fatty acids.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 214


Design Notes

L-FABP is expressed in rat liver, and we optimized its codon usage to that of E. coli for DNA synthesis.


Source

it is a synthetic coding sequence.

References

[1] Frolov. A, Cho. TH and Murphy EJ. Isoforms of rat liver fatty acid binding protein differ in structure and affinity for fatty acids and fatty acyl CoAs. Biochemistry,[J], 1997 May; 36(21)

[2] Tony Velkov,Sara Chuangand Richard Prankerd.An improved method for the purification of rat liver-type fatty acid binding protein from Escherichia coli.Protein Expr Purif,[J],2005Nov;44(1):23–31.