Protein_Domain
LPP-ompA

Part:BBa_K2302003:Design

Designed by: Hongyang Wei   Group: iGEM17_NEFU_China   (2017-10-19)
Revision as of 02:51, 20 October 2017 by Weihongyang (Talk | contribs) (→‎References)

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Lpp-ompA,a peptide that can anchor its fused gene product to the outer membrane.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 433
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the peptide is widely used to express various proteins to be anchored on bacterial membrane, such as GFP and fatty acid binding protein.


Source

it is a synthetic coding sequence based on the biased codons of E. coli.

References

[1]Francisco JA,Earhart CF,Georgiou G.Transport and anchoring of beta-lactamase to the external surface of Escherichia coli.Proc Natl Acad Sci USA.[J],1992,89(7):2713-2717.