Part:BBa_K2483009

IAAM and IAAH yeast optimized under GAL1 control

This part was used as a control for our liquid-liquid phase separation project. We used the CDS of the Indoleacetic acid-producing enzymes IAAM and IAAH from the part BBa_K515100 by Imperial College 2011 and codon optimized them for S.cerevisiae.

As a promoter, we used GAL1 (strong, galactose-induced promoter) and CYC1 as a terminator.

The CDS' are facing away from each other because of the way this part was assembled (we used Gibson Assembly) and the original structure of the plasmid (pYES2/CT).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 2301
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 2301
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 2301
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 2301
  Illegal NgoMIV site found at 1212
  Illegal AgeI site found at 916
  Illegal AgeI site found at 2086
  Illegal AgeI site found at 2377
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 3594
  Illegal BsaI.rc site found at 913