DNA

Part:BBa_K2325003

Designed by: Huixian Li   Group: iGEM17_SCUT-FSE-CHINA   (2017-10-15)
Revision as of 16:02, 19 October 2017 by KahiuLau (Talk | contribs)


T7 phage N20-sgRNA

The BBa_K2325003 is a DNA sequence contains N20 associated with sgRNA. The N20-1 is selected from the genome of T7 phage, which is responsible for the synthesis of the DNA packaging protein during phage infection. This part confers resistance against T7 phage upon a strain containing the Cas9 and sgRNA parts. This part can be considered as an improvement of the (BBa_K1129012), as they contain same N20 sequence but different sgRNA sequence. The way we constructed the part also promoted the function of resistance against T7 phage.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None