Part:BBa_K2273033

sfGFP

The Codon Adaptation Index (CAI) proposed by Sharp and Li (1987)53 was used to quantify the adaption of FP-encoding genes to the B. subtilis codon usage. For CAIBSU, codon frequencies were compared to those obtained from the Kazusa codon usage database, which is based on the analysis of all B. subtilis genes regardless of their expression levels54. The CAI was calculated using a customized version of the AutoAnnotator created by the iGEM Team TU-Munich (2013)55. The FP-encoding genes were synthesized by GeneArt® and marked by addition of “BSU” to the protein name (except of GFPmut1, for which the optimized variant is marked by the addition of “LT”, because the LifeTech® codon adaptation algorithm was used). For sfGFP we used the Streptococcus pneumoniae codon adapted version, which was previously described to work best for B. subtilis47.

Single copy Constitutive promoter (Pveg) 2012 verlinken iGEM lmu 2012 BBa_K823003

Overkamp, W. et al. Benchmarking various green fluorescent protein variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for live cell imaging. Appl. Environ. Microbiol. 79, 6481–6490 (2013).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]