Coding

Part:BBa_K2255000:Design

Designed by: Jeremy Cartalas   Group: iGEM17_Aix-Marseille   (2017-08-28)
Revision as of 15:05, 19 October 2017 by Kamy (Talk | contribs) (→‎Design Notes)


Enoyl-CoA hydratase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1093
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 16


Design Notes

The Enoyl-CoA hydratase DspI [1] is necessary to the production of 2-cis-decenenoic acid by Pseudomonas aeruginosa, so we search its DNA sequence in the genome to create our first biobrick. This enzyme gene is on a operon along the other gene crucial for the formation of 2-cis-decenenoic acid.

We took the gene coding for the right enzyme and optimized its sequence for a production in E.coli. Then a Rfc10 prefix and sufix was added.

Source

It comes from the genome of Pseudomonas aeruginosa.

References

  1. ↑ Amari, D. T., Marques, C. N. H. & Davies, D. G. The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis-2-Decenoic Acid. J. Bacteriol. 195, 4600–4610 (2013).