Coding

Part:BBa_K2507000

Designed by: Xiaoxiao Chen   Group: iGEM17_SHSBNU_China   (2017-10-01)
Revision as of 12:54, 19 October 2017 by HaoyuZhou (Talk | contribs)

J23109-ttrR-PttrB185-BBa_K1033919

Background

ThsS/R are two basic parts which belong to the two-component systems. This system is from marine Shewanella species. After validate this system in laboratory Escherichia coli, this system can function as a thiosulfate sensor and reporter. Because of thiosulfate is an indicator of gut inflammation, this system can be used as a sensor of gut inflammation. Combining with sfGFP or chromoprotein， after receive the sign from thsS, this system can finally repress the result by produce protein.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1078
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 477
    Illegal BamHI site found at 525
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1010



Results



[edit]
Categories
Parameters
None