Coding

Part:BBa_K2507000

Designed by: Xiaoxiao Chen   Group: iGEM17_SHSBNU_China   (2017-10-01)
Revision as of 12:53, 19 October 2017 by HaoyuZhou (Talk | contribs)

J23109-ttrR-PttrB185-BBa_K1033919

Background

ThsS/R are two basic parts which belong to the two-component systems. This system is from marine Shewanella species. After validate this system in laboratory Escherichia coli, this system can function as a thiosulfate sensor and reporter. Because of thiosulfate is an indicator of gut inflammation, this system can be used as a sensor of gut inflammation. Combining with sfGFP or chromoprotein， after receive the sign from thsS, this system can finally repress the result by produce protein.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 318
  • 1000
    COMPATIBLE WITH RFC[1000]



Results



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Categories
Parameters
None