Coding
rISG65

Part:BBa_K2387061

Designed by: Linda van Oosten   Group: iGEM17_Wageningen_UR   (2017-10-19)
Revision as of 12:47, 19 October 2017 by Registry (Talk | contribs)

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Double tagged ISG65 of T.b. gambiense

This part contains the extracellular domain (domain 21-385) of the invariant surface glycoprotein 65 (ISG65) of Trypanosoma brucei gambiense. This parasite causes African Sleeping Sickness (Human African Trypanosomiasis). ISG65 is a surface protein and thus antigen of this organism. It is double tagged, having a Strep-tag II and 10xHis tag for the convenience of purification. Moreover, because of two distinct cleavage sites, the tags can be cleaved of if necessary. By only taking the extracellular domain, protein expression and purification are simplified since a soluble protein is formed. Note that this DNA originates from parasitic genomic DNA, and codon optimization has not been performed. Hence, it is advised to use an E. coli strain optimized for this. This protein can be used for, for example, a proof-of-principle for the binding to or detection of African Sleeping Sickness.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 823
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None