Coding

Part:BBa_K2380041

Designed by: Daniel Kelvin, Cristina Kurzknabe, Patrick Müller, Elena Nickels, Bea Spiekermann, Lara Steinel   Group: iGEM17_TU_Darmstadt   (2017-10-19)
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Chitin deacetylase NodB

Chitin deacetylase (CDA) NodB from Sinorhizobium meliloti. The enzyme is a hydrolase and deacetylates chitin to chitosan. It solely deacetylates the first position of the non-reducing end in a chitin oligomer. We used it for the production of chitosan with a defined deacetylation pattern. The CDA NodB forms inclusion bodies in E.coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None