Coding
Part:BBa_K2380041
Designed by: Daniel Kelvin, Cristina Kurzknabe, Patrick Müller, Elena Nickels, Bea Spiekermann, Lara Steinel Group: iGEM17_TU_Darmstadt (2017-10-19)
Chitin deacetylase NodB
Chitin deacetylase (CDA) NodB from Sinorhizobium meliloti. The enzyme is a hydrolase and deacetylates chitin to chitosan. It solely deacetylates the first position of the non-reducing end in a chitin oligomer. We used it for the production of chitosan with a defined deacetylation pattern. The CDA NodB forms inclusion bodies in E.coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |