Protein_Domain

Part:BBa_K2356000

Designed by: Ralf Philipsen   Group: iGEM17_TU-Eindhoven   (2017-10-19)
Revision as of 08:37, 19 October 2017 by Registry (Talk | contribs)

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CT33 with mCherry and Strep-tag II

The sequence starts with DNA coding for mCherry, a fluorescent protein. This is followed by DNA coding for Strep-tag II, allowing it to bind to Strep-Tactin or other Streptavidin variants. The last part encodes for CT33, a domain comprising the final 33 amino acids of the C-terminus of H+-ATPase, a known binding partner of 14-3-3 scaffolds. The parts are connected via linkers, consisting mostly of Glycine and Serine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 22
    Illegal BamHI site found at 751
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 772
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None