Composite

Part:BBa_K2250007

Designed by: Shuailin Li   Group: iGEM17_Tsinghua-A   (2017-10-19)
Revision as of 08:09, 19 October 2017 by Shuailin10 (Talk | contribs)


Pconst-LuxI-Pconst-GFP

This is the secretion part of warrior 2 in our project. RhlI make the warrior 2 able to secrete the 3OC6HSL to the medium and then enter the other cell and kill warrior 1/beggar 1/farmer 1. This can be realized by 3OC6HSL binding to LuxR, activating the expression of LacI, and then LacI inhibiting the expression of CmR, making the warrior 1/beggar 1/farmer 1 unable to survive under the Chloramphenicol. Also, the GFP is used to distinguish between group 1 (containing warrior 1/beggar 1/farmer 1) and group2 (containing warrior 2/beggar 2/farmer 2). GFP represents group 2.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 856
    Illegal NheI site found at 879
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 698
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1554


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Categories
Parameters
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