DNA

Part:BBa_K2514000:Design

Designed by: Rui Zhou   Group: iGEM17_Worldshaper-Wuhan   (2017-10-19)
Revision as of 06:11, 19 October 2017 by Ruizhou (Talk | contribs) (References)


pSB1C3-miR-21-sponge-6s


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Pervious studies indicated sponges containing bulged sites that are mispaired opposite miRNA are more effective than perfectly complementary sponges for individual microRNAs. And different miR-21 sponges contained different binding sites may have different accuracy for measuring the expression of miR-21 in colorectal cancer cells. So we desinged two different mir-21 sponges with different binding sits to test the efficiency of sponges. One mir-21 sponge contains six miR-21 binding sites with 3-nt spacers for bulged sites. We wanted to compare the accuracy and the effectiveness of different miR-21 sponges contained different binding sites for measuring the expression of miR-21 in colorectal cancer cells.


Source

We designed mir-21 sponges contains six miR-21 binding sites with 3-nt spacers for bulged sites based on the sequence of hsa-miR-21 according to the previous study (Ebert MS, Neilson JR, Sharp PA. MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells. Nat Methods. 2007 Sep;4(9):721-6)

References

Ebert MS, Neilson JR, Sharp PA. MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells. Nat Methods. 2007 Sep;4(9):721-6 Ebert MS, Sharp PA. MicroRNA sponges: progress and possibilities. RNA. 2010 Nov;16(11):2043-50.