Coding

Part:BBa_K2332021

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-18)
Revision as of 23:26, 18 October 2017 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Blue light repressible bioluminescence (Pblrep LuxCDABE )

Our LIT Bulb will be composed of light sensitive E. coli that can detect sunlight (blue light) to repress bioluminescence (LuxCDABE). In the presence of light transcription of the LuxCDABE for the production of Luciferase and substrates will be inhibited. In the dark, the inhibition will be released and bioluminescence will be induced. This part encodes the necessary requirements for bioluminescence production, codon optimized for E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2621
    Illegal AgeI site found at 2888
    Illegal AgeI site found at 3416
    Illegal AgeI site found at 3997
    Illegal AgeI site found at 4444
    Illegal AgeI site found at 5322
    Illegal AgeI site found at 5537
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None