Regulatory
Part:BBa_K2332019
Designed by: Paola Handal Group: iGEM17_UCL (2017-10-18)
Blue light repressible promoter (PBLrep)
Our LIT Bulb will be composed of light sensitive E. coli that can detect sunlight (blue light) to repress bioluminescence (LuxCDABE) using this promoter part. In the presence of light transcription of the LuxCDABE for the production of Luciferase and substrates will be inhibited. In the dark, the inhibition will be released and bioluminescence will be induced. This part encodes the necessary requirements for bioluminescence production, codon optimized for E. coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |