Composite

Part:BBa_K2209002

Designed by: Qinyu Ge   Group: iGEM17_Nanjing_NFLS   (2017-10-18)
Revision as of 13:24, 18 October 2017 by Registry (Talk | contribs)

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Fused strain

The function of modified gene verification should be performed. In this present study, the combined plasmid was transferred to expression strain P.putida KT2440 through triphilicity. Donor strain was E.coli DH-5α, E.coli HB101（pRK2013）act as auxiliary strains and recipient bacterium was P.putida KT2440. The fuse bacterium was further using to carry out the verification experiment, it showed higher expression level and could degrade aniline analogue high-efficiency.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 165
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1134
    Illegal BsaI.rc site found at 1720
    Illegal SapI site found at 4799


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