Reporter

Part:BBa_I746105

Designed by: Zhizhen Zhao   Group: iGEM07_Cambridge   (2007-09-07)
Revision as of 12:47, 18 October 2017 by Huqiwen (Talk | contribs)


GFP with agr P2 promoter

This is composed of a promoter and a GFP reporter gene. This can produce green fluorescence and in theory the strength of the green flourence is much stronger when there is phosphorylated AgrA in the cell. This is used to test the basal activity of agr P2 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 769


Contribution：TMMU-China 2017

Author：Yizhen Xu Summary：In this contribution, we characterized this part in S.aureus.

Characterization in S.aureus

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