Part:BBa_I751000

pBR322 (Pst I site mutated)

pBR322 has Pst I site in its Amp resistant gene. It prevents us from introducing Biobrick site(Pst I included) to this plasmid. For that reason, we introduced a silent mutation at the site, in order not to restrict Amp resistant gene. The mutation is A546C(DNA sequence) in the Amp resistant gene. Introducing this mutation allowed us to aim at constructing pBR standard plasmid.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 229
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 375
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 401
  Illegal NgoMIV site found at 769
  Illegal NgoMIV site found at 929
  Illegal NgoMIV site found at 1283
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 3433
  Illegal SapI site found at 2350