Translational_Unit

Part:BBa_K2387052

Designed by: José Manuel Salvador López   Group: iGEM17_Wageningen_UR   (2017-10-18)
Revision as of 10:02, 18 October 2017 by Registry (Talk | contribs)

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mCerulean controlled by inducible araC/pBAD promoter

mCerulean is a bright cyan monomeric fluorescent protein. In this composite, its expression is regulated by the araC/pBad promoter and by a strong RBS. mVenus presents a maximum pick of excitation at 433 nm and a maximum pick of emission at 475 nm. mCerulean was engineered from eCFP to grant its fluorescence more intensity and more lifetime.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
//cds/reporter
//cds/reporter/cfp
//chassis/prokaryote
//function/reporter/fluorescence
Parameters
absMax. 433 nm
chassisE. coli
colorCyan Fluorescence, Bright Yellow cells
emissionMax. 475 nm