Reporter

Part:BBa_K2450301:Design

Designed by: Zoe Ford   Group: iGEM17_Oxford   (2017-10-17)
Revision as of 19:03, 17 October 2017 by Registry (Talk | contribs)

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pTet promoter coding eYFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We are using eYFP as it is very bright and detectable in low quantities, and also compatible for detection with CFP and mCherry. The sequence for eYFP comes from AddGene.


Source

This part uses the Tet operator from BBa_R0040, which is a medium strength promoter. eYFP from AddGene

References