Device

Part:BBa_K2265000

Designed by: Jenny Nesje   Group: iGEM17_NTNU_Trondheim   (2017-10-17)
Revision as of 12:11, 17 October 2017 by Registry (Talk | contribs)

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MP6 mutator (original)

A mutator operon controlled by a pBAD promoter, which also contains the required AraC gene. Contains 6 genes pertaining to mutagenesis. Under arabinose induction it increases the mutational frequency of the host. Repressed by glucose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1413
    Illegal BamHI site found at 2305
    Illegal XhoI site found at 3889
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1285
    Illegal AgeI site found at 1774
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4562
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None