Composite

Part:BBa_K2324011

Designed by: Rahan Nazeer   Group: iGEM17_Exeter   (2017-10-16)
Revision as of 20:08, 16 October 2017 by Registry (Talk | contribs)

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T7_FimH_225sfGFP

This part synthesises a FimH adhesin protein fused with GFP at its 225th amino acid residue. The coding sequence is under the control of an IPTG-inducible, T7 promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, produces a fluorescent FimH protein that should involve itself in pilus biosynthesis when co-transformed with a plasmid containing the fim operon .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 451
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None