Coding

Part:BBa_K2324003:Design

Designed by: Rahan Nazeer   Group: iGEM17_Exeter   (2017-10-12)
Revision as of 19:12, 16 October 2017 by Rahannazeer (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


FimH+sfGFP at residue 258


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimisation for expression in E. coli . Research was required to identify the ideal placement of the foreign protein domain.


Source

The FimH sequence is a codon optimised version of the registry entry BBa_K1850001. The sfGFP sequence is a codon optimised version of the registry entry BBa_I746916.

References