Part:BBa_K2332014
Blue light inducible expression of Intimin'-SpyCatcher (Pblind Intimin'-SpyCatcher)
To trigger light inducible cell adhesion, we decided to use our blue light inducible promoter to trigger the synthesis of either SpyTag or SpyCatcher, two binding partners that form rapid covalent bonds. SpyTag (13 amino acids) and SpyCatcher (138 amino acids, 15 kDa) come from CnaB2 (immunoglobulin-llike collagen adhesin domain) of the FbaB protein, found in the invasive strains of S. pyogenes. We decided to fuse SpyTag and SpyCatcher to outer membrane surface proteins in distinct cell populations that would then aggregate once mixed together. This biobrick is the fusion of a truncated version of Intimin and SpyCatcher for the cell surface display of SpyCatcher under the control of a blue light inducible promoter.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 898
Illegal NgoMIV site found at 1639 - 1000COMPATIBLE WITH RFC[1000]
None |