Composite

Part:BBa_K2332011:Design

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-16)
Revision as of 16:48, 16 October 2017 by Registry (Talk | contribs)

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Blue light inducible expression of Intimin'-SpyTag (Pblind Intimin'-SpyTag)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 880
    Illegal NgoMIV site found at 1621
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We decided to fuse SpyCatcher to the N-terminal of a truncated version of Intimin as this has been previously proven to function effectively for cell surface display of up to 30kDa passenger proteins


Source

All parts were obtained as synthetic gBlocks Intimin protein sequence obtained from: http://www.uniprot.org/uniprot/P43261#sequences And reverse translated using: http://www.bioinformatics.org/sms2/rev_trans.html Intimin for the cell surface display of our tag was truncated according to Wentzel et al., 2001 SpyTag: BBa_K1159201 Pblind:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5001607/

References