Composite
Part:BBa_K2332011:Design
Designed by: Paola Handal Group: iGEM17_UCL (2017-10-16)
Blue light inducible expression of Intimin'-SpyTag (Pblind Intimin'-SpyTag)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 880
Illegal NgoMIV site found at 1621 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We decided to fuse SpyCatcher to the N-terminal of a truncated version of Intimin as this has been previously proven to function effectively for cell surface display of up to 30kDa passenger proteins
Source
All parts were obtained as synthetic gBlocks Intimin protein sequence obtained from: http://www.uniprot.org/uniprot/P43261#sequences And reverse translated using: http://www.bioinformatics.org/sms2/rev_trans.html Intimin for the cell surface display of our tag was truncated according to Wentzel et al., 2001 SpyTag: BBa_K1159201 Pblind:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5001607/