Composite

Part:BBa_K2387032:Design

Designed by: Bart Scholten   Group: iGEM17_Wageningen_UR   (2017-10-16)
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CpxR-eYFPn[1-154] and CpxR-eYPFc[155-238] + araC/pBAD promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 1283
    Illegal XhoI site found at 2498
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1694
    Illegal AgeI site found at 2909
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

A long flexible linker was used to fuse CpxR and eYFPc to maximize the availability of eYFPc[155-238] and thus increase the chances to reassemble with eYFPn[1-154]. Both fusions were put under control of one promoter and the same RBS to ensure similar levels of protein expression.


Source

eYFP originates from the jellyfish Aequorea victoria

araC/pBAD is an endogenous E. coli promoter

These original parts are taken from the iGEM repository; [http://parts.igem.com/BBa_E0030 eYFP] [http://parts.igem.com/BBa_I0030 araC/pBAD promoter] [http://parts.igem.com/BBa_B0034 RBS BBa_B0034] [http://parts.igem.com/Part:BBa_K1486004 Flexible Linker] [http://parts.igem.com/Part:BBa_K2387002 CpxR]

eYFPn[1-154] and eYFPc[155-238] were created in during [http://2017.igem.org/Team:Wageningen_UR Wageningen_URs 2017 iGEM project].


References