Composite

Part:BBa_K2387030

Designed by: Bart Scholten   Group: iGEM17_Wageningen_UR   (2017-10-16)
Revision as of 12:35, 16 October 2017 by Bart scholten (Talk | contribs)


CpxR-eYFPc fusion + araC/pBAD promoter

The [http://parts.igem.com/Part:BBa_K2387006 C-terminus of eYFP] is fused to Cpx response regulator [http://parts.igem.com/Part:BBa_K2387002 CpxR] in order to visualize the activation of the Cpx pathway. The fusion is put under the control of the L-arabinose inducible araC/pBAD promoter. Strong RBS BBa_B0034 is used to regulate transcription.

CpxR and eYFPn were linked together using a [http://parts.igem.com/Part:BBa_K1486004 Flexible Linker] consisting of two times the amino acids GGGGS.

It can be used in combination with the fusion [http://parts.igem.com/Part:BBa_K2387011 CpxR-eYFPc[155-238]] to visualize the activation of the Cpx pathway via Bimolecular Fluorescence Complementation by using the CpxA-CpxR interaction. Furthermore, it is used as a negative control in visualizing the Cpx pathway by combining BiFC to CpxR dimerization [http://parts.igem.com/Part:BBa_K2387032 CpxR-eYFPn[1-154]-CpxR-eYFPc[155-238]]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 1283
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1694
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


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Categories
Parameters
None