Composite

Part:BBa_K2404020:Design

Designed by: Ryan Coates   Group: iGEM17_Cardiff_Wales   (2017-10-16)
Revision as of 11:20, 16 October 2017 by Registry (Talk | contribs)

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TSH antagonist under control of the PR2 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1045
    Illegal PstI site found at 1087
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 242
    Illegal PstI site found at 1045
    Illegal PstI site found at 1087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1300
    Illegal XhoI site found at 1112
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1045
    Illegal PstI site found at 1087
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1045
    Illegal PstI site found at 1087
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was put together using Golden Gate cloning, so has the appropriate restriction enzyme recognition sites in it. It is flanked by Type IIS restriction endonuclease recognition sites.


Source

The CDS was synthesised, the promoter isolated from Arabidopsis thaliana , and the terminator isolated from Agrobacterium tumefaciens

References