Composite

Part:BBa_K2404018

Designed by: Ryan Coates   Group: iGEM17_Cardiff_Wales   (2017-10-16)
Revision as of 11:17, 16 October 2017 by Registry (Talk | contribs)

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TSH antagonist under control of the PDF1.2 promoter

A thyroid stimulating hormone antagonist, with His-tags for purification, under control of the jasmonic acid-inducible promoter, PDF1.2. The terminator is the noplaine synthase gene terminator, native to Agrobacterium tumefaciens . This construct in contained within the pGB-A2 plasmid. It should be used to express and create the TSH antagonist in the presence of jasmonic acid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1048
    Illegal PstI site found at 1090
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1048
    Illegal PstI site found at 1090
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 412
    Illegal BamHI site found at 1303
    Illegal XhoI site found at 1115
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1048
    Illegal PstI site found at 1090
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1048
    Illegal PstI site found at 1090
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None