Composite

Part:BBa_K2404016:Design

Designed by: Ryan Coates   Group: iGEM17_Cardiff_Wales   (2017-10-16)
Revision as of 11:11, 16 October 2017 by Registry (Talk | contribs)

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A TSH antagonist with His-Tags, controlled by the LexA promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 22
    Illegal PstI site found at 888
    Illegal PstI site found at 930
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 22
    Illegal PstI site found at 888
    Illegal PstI site found at 930
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1143
    Illegal XhoI site found at 955
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 22
    Illegal PstI site found at 888
    Illegal PstI site found at 930
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 22
    Illegal PstI site found at 888
    Illegal PstI site found at 930
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

These parts were put together using Golden Gate cloning, and have appropriate restriction enzyme recognition sites as a result. Type IIS recognition sites flank the construct.


Source

The CDS was synthesised, and the promoter and terminator isolated from gDNA of E. coli and Agrobacterium tumefaciens , respectively.

References