Composite

Part:BBa_K2404014

Designed by: Ryan Coates   Group: iGEM17_Cardiff_Wales   (2017-10-16)
Revision as of 11:07, 16 October 2017 by Registry (Talk | contribs)

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Luc+ gene under control of the LexA promoter

The luciferase firefly gene under control of the inducible promoter of the LexA gene, native to E. coli . This promoter is induced by XVE. This construct has the terminator from the Nopaline synthase gene, native to Agrobacterium tumefaciens . This construct is contained within the pGB-A2 plasmid. It should be used as an a construct to test the promoter strength of the LexA promoter, both with and without the presence of XVE.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 15
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1171
    Illegal BamHI site found at 1017
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 15
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 15
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None