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Part:BBa_K2387004:Design

Designed by: Bart Scholten   Group: iGEM17_Wageningen_UR   (2017-10-15)
Revision as of 11:37, 15 October 2017 by Registry (Talk | contribs)

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eYFP N-terminus controlled by inducible araC/pBAD promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

Double stop codon (TAATAA) added


Source

eYFP originates from A. victoria araC/pBAD promoter originates from E. coli All parts taken from the repository

References