DNA

Part:BBa_K2429016:Design

Designed by: Adil Yusuf   Group: iGEM17_MIT   (2017-10-14)
Revision as of 02:58, 15 October 2017 by Registry (Talk | contribs)

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mKate-FF4 Reporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 540
    Illegal EcoRI site found at 1157
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 540
    Illegal EcoRI site found at 1157
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 540
    Illegal EcoRI site found at 1157
    Illegal BamHI site found at 1
    Illegal XhoI site found at 437
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 540
    Illegal EcoRI site found at 1157
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 540
    Illegal EcoRI site found at 1157
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1062
    Illegal SapI.rc site found at 55


Design Notes

Ensure that the intron can be efficient spliced out of the construct.


Source

N/A

References