Coding

Part:BBa_K2380000

Designed by: Lisa-Marie Brenner, Isabelle Feinauer, Werner Kleindienst, Cristina Kurzknabe, Isabelle Marquardt, Patrick Müller, Sven Storch   Group: iGEM17_TU_Darmstadt   (2017-10-14)
Revision as of 16:40, 14 October 2017 by Th.wagner (Talk | contribs)


Chitin synthase NodC from Rhizobium leguminosarum

The Chitin Synthase (CHS) NodC from Rhizobium leguminosarum is an N-acetylglucosaminyl transferase which catalyzes the formation of chitin pentamers by using UDP-acetylglucosamine as donor and N-acetylglucosamine as acceptor.

1. Usage and Biology

Besides cellulose, chitin is the most common natural polysaccharide in nature. Chitin is composed of β(1,4) linked 2-acetamido-2-deoxy-β-D-glucose (N-acetylglucosamine). The polymer is a component of fungi cell walls and the exoskeletons of insects and crustaceans, like crabs or shrimps [Dutta et al., 2004; Kumar, 2000]. The extraction of chitin from crustaceans produces a lot of waste and uses a lot of chemicals. But bacteria, like E. coli can produce chitin via a chitin synthase (CHS) in an environmentally friendly manner. The production of chitin appears to be important as it is a useful substance which finds applications in medicinal, industrial and biotechnological research. Chitin, and its derivate chitosan, is non-toxic, biocompatible and biodegradable. Their bioactivities are for example the promotion of wound healing or hemostatic activity, immune enhancement, eliciting biological responses, and antimicrobial activity [Kurita, 2006]. The nodC gene is originating from the gram-negative bacterium Rhizobium leguminosarum and is a homologue to the chitin synthase from yeast. Rhizobium species live in symbiosis with legumes, where the bacteria form nitrogen-fixing nodules in the legume roots. This interaction leads to an activation of the bacterial nodulation (nod) genes and the secretion of Nod factors. nodC belongs to these nod genes which create and modify the Nod factors. The NodC protein has strongly hydrophobic domains which indicate that it is an integral or transmembrane protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 373
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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