Tag
Part:BBa_K2368009:Design
Designed by: BIT-China 2017 Group: iGEM17_BIT-China (2017-10-02)
His+T1R2 overlap
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Firstly , we construct the specific primer that consists of the gene sequence of HIS tag. Then, we combine T1R2 with the HIS by the method of PCR.