DNA

Part:BBa_K2429012:Design

Designed by: Nia Myrie   Group: iGEM17_MIT   (2017-10-13)
Revision as of 01:10, 14 October 2017 by Registry (Talk | contribs)

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pENTR Ms2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 294
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 294
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 294
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 294
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 294
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

human codon optimized


Source

This part was obtained from another researcher who was kind enough to give us the sequence and physical plasmid containing the protein with the DDX6 domain sequence attached to the Ms2 sequence.

References