Part:BBa_K2429011
pENTR L. shahii NL-dCas13a-NL
Leptotrichia shahii deactivated Cas13a protein coding region. This part produces a deactivated version of the CRISPR protein Cas13a, which normally binds and cuts mRNA. With the deactivation sequence, the protein becomes catalytically inactive, and unable to cut the mRNA; however, it can still bind to the mRNA molecule. Flanking the dCas13a coding sequence are a pair of nuclear localization sequences (NLSs) The sequences code for signaling peptides that indicates that the remaining portion of the translated protein will remain in the cell (specifically in the nucleus) rather then get excreted by the cell. The translated amino acid sequence of the NLS is PKKKRRV and classifies as a classic nuclear localization sequence (cNLS). Such sequences are considered the best characterized transport signal [1].
[1] Lange, et al. "Classical Nuclear Localization Signals: Definition, Function, and Interaction with Importin α*"
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 4422
Illegal PstI site found at 1903
Illegal PstI site found at 2272
Illegal PstI site found at 3001 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4422
Illegal PstI site found at 1903
Illegal PstI site found at 2272
Illegal PstI site found at 3001 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4422
Illegal BglII site found at 647
Illegal BglII site found at 1271
Illegal BglII site found at 1667
Illegal BglII site found at 1958
Illegal BglII site found at 2048
Illegal BglII site found at 3209
Illegal BglII site found at 3296
Illegal BamHI site found at 1
Illegal BamHI site found at 70
Illegal BamHI site found at 4291 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 4422
Illegal PstI site found at 1903
Illegal PstI site found at 2272
Illegal PstI site found at 3001 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 4422
Illegal PstI site found at 1903
Illegal PstI site found at 2272
Illegal PstI site found at 3001
Illegal NgoMIV site found at 4248
Illegal NgoMIV site found at 4267 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 633
Illegal SapI.rc site found at 739
Illegal SapI.rc site found at 1609
Illegal SapI.rc site found at 2443
protein |