DNA

Part:BBa_K2429010

Designed by: Nia Myrie   Group: iGEM17_MIT   (2017-10-13)
Revision as of 19:29, 13 October 2017 by Registry (Talk | contribs)

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pENTR L. shahii NL-Cas13a-NL

Leptotrichia shahii Cas13a protein coding region. This part produces a the CRISPR protein Cas13a, which binds and cuts mRNA. Upon recognition of the mRNA, this protein exhibits "promiscuous" ribonuclease activity, and cuts at random base pairs along the RNA molecule. Flanking the Cas13a coding sequence are a pair of nuclear localization sequences (NLSs) The sequences code for signaling peptides that indicates that the remaining portion of the translated protein will remain in the cell (specifically in the nucleus) rather then get excreted by the cell. The translated amino acid sequence of the NLS is PKKKRRV and classifies as a classic nuclear localization sequence (cNLS). Such sequences are considered the best characterized transport signal [1].

[1] Lange, et al. "Classical Nuclear Localization Signals: Definition, Function, and Interaction with Importin α*"

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4425
    Illegal PstI site found at 1903
    Illegal PstI site found at 2272
    Illegal PstI site found at 3001
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4425
    Illegal PstI site found at 1903
    Illegal PstI site found at 2272
    Illegal PstI site found at 3001
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4425
    Illegal BglII site found at 647
    Illegal BglII site found at 1271
    Illegal BglII site found at 1667
    Illegal BglII site found at 1958
    Illegal BglII site found at 2048
    Illegal BglII site found at 3209
    Illegal BglII site found at 3296
    Illegal BamHI site found at 1
    Illegal BamHI site found at 70
    Illegal BamHI site found at 4291
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4425
    Illegal PstI site found at 1903
    Illegal PstI site found at 2272
    Illegal PstI site found at 3001
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4425
    Illegal PstI site found at 1903
    Illegal PstI site found at 2272
    Illegal PstI site found at 3001
    Illegal NgoMIV site found at 4248
    Illegal NgoMIV site found at 4267
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 633
    Illegal SapI.rc site found at 739
    Illegal SapI.rc site found at 1609
    Illegal SapI.rc site found at 2443


[edit]
Categories
//cds
Parameters
protein