Part:BBa_K2387001

Monomeric chromoprotein anm2CP controlled by inducible araC/pBAD promoter

In this composite, the coding sequence for anm2CP is under the control of the inducible pBad/araC promoter (BBa_I0500), as well as a strong RBS (BBa_B0034).

Usage and Biology

anm2CP is a monomeric chromoprotein (BBa_K2387000), the only one that is known to be produced in nature. The sequence can be retrived from the genome of the jellyfish Anthomedusae sp. SL-2003 (GenBank, AY485336). It shows a bright pink color when expressed to sufficient concentrations. Its absorbance spectrum shows a pick with a maximum at 572 nm. As other fluorescent proteins and chromoproteins, it has a structure similar to that of GFP, as it can be observed from the structure of KillerRed (PSB 2WIQ) a fluorescent protein evolved from anm2CP. Most chromoproteins are tetrameric, which makes them a bad candidate for protein fusions and other applications. Among other applications, anm2CP would be able to be split in order to be used in bimolecular complementation techniques.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1205
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1280
  Illegal BamHI site found at 1144
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 979
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 961