Composite

Part:BBa_K2197300

Designed by: Ip Wang Chak   Group: iGEM17_Hong_Kong_UCCKE   (2017-08-11)
Revision as of 12:55, 13 October 2017 by Hsiuowen81 (Talk | contribs)


Expression of GFP under the control of a uric acid concentration-sensitive promoter device

Engineered e.coli encodes part BBa_K2197300. By adding blood samples to culture medium which engineered e.coli is cultured, e.coli expresses different level of GFP. By analysing the GFP's fluorescence level with a plate reader, the concentration of uric acid can be determined. This part ensures a rapid detection of uric acid concentration thus gout.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1098
    Illegal NheI site found at 1121
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 555
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 291
    Illegal BsaI.rc site found at 1814
    Illegal SapI.rc site found at 255


[edit]
Categories
Parameters
None