Part:BBa_K2429007

pENTR L. shahii dCas13a

Leptotrichia shahii deactivated Cas13a protein coding region. This part produces a deactivated version of the CRISPR protein Cas13a, which normally binds and cuts mRNA. With the deactivation sequence, the protein becomes catalytically inactive, and unable to cut the mRNA; however, it can still bind to the mRNA molecule.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 294
  Illegal PstI site found at 1894
  Illegal PstI site found at 2263
  Illegal PstI site found at 2992
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 294
  Illegal PstI site found at 1894
  Illegal PstI site found at 2263
  Illegal PstI site found at 2992
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 294
  Illegal BglII site found at 638
  Illegal BglII site found at 1262
  Illegal BglII site found at 1658
  Illegal BglII site found at 1949
  Illegal BglII site found at 2039
  Illegal BglII site found at 3200
  Illegal BglII site found at 3287
  Illegal BamHI site found at 1
  Illegal BamHI site found at 4282
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 294
  Illegal PstI site found at 1894
  Illegal PstI site found at 2263
  Illegal PstI site found at 2992
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 294
  Illegal PstI site found at 1894
  Illegal PstI site found at 2263
  Illegal PstI site found at 2992
  Illegal NgoMIV site found at 4239
  Illegal NgoMIV site found at 4258
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 624
  Illegal SapI.rc site found at 730
  Illegal SapI.rc site found at 1600
  Illegal SapI.rc site found at 2434