RNA

Part:BBa_K2246006

Designed by: JUAN CARLOS RUEDA SILVA   Group: iGEM17_TecCEM   (2017-10-02)
Revision as of 18:46, 12 October 2017 by JRUEDA (Talk | contribs)

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anti-GFP siRNA

siRNA sequence for silencing protein green flourescent protein (GFP) gene. This sequence must be used as dsRNA, in this way it works as a small interfering RNA specific for green flourescent protein (GFP) gene. Designed to be used as negative control during RNA interference experiments. In previous studies involving RNA interferance in D.citri, a dsRNA for GFP was used as negative control, see Taning, Andrade, Hunter, Christiaens & Smagghe (2016), El-Shesheny, Hajeri, El-Hawary, Gowda & Killiny (2013) and Yu, Gowda & Killiny (2017). El-Shesheny I, Hajeri S, El-Hawary I, Gowda S, Killiny N .(2013). Silencing Abnormal Wing Disc Gene of the Asian Citrus Psyllid, Diaphorina citri Disrupts Adult Wing Development and Increases Nymph Mortality. PLoS ONE 8(5): e65392. doi:10.1371/journal.pone.0065392 Taning C., Andrade E., Hunter W., Christiaens O. & Smagghe G. (2016). Asian Citrus Psyllid RNAi Pathway –RNAi evidence. Scientific Reports. DOI: 10.1038/srep38082 Yu X., Gowda S. & Killiny N. (2017). Double-stranded RNA delivery throughsoaking mediates silencing of the muscleprotein 20 and increases mortality to the Asiancitrus psyllid, Diaphorina citri. Pest Management Science. DOI 10.1002/ps.4549


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None