Composite
Part:BBa_K2332005:Design
Designed by: Paola Handal Group: iGEM17_UCL (2017-10-12)
EL222, photosensitive DNA-binding protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 142
Illegal AgeI site found at 367 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We had to reverse translate and codon optimize the protein sequence for its usage in E. coli and ensure no illegal sites were present.
Source
The reverse translation of EL222 (UniProtKB/Swiss-Prot: Q2NB98.1) optimized for E. coli was obtained as a synthetic gBlock along with the promoter, RBS and terminator sequences.