Composite

Part:BBa_K2447016

Designed by: Chee Wai Kit (david)   Group: iGEM17_NUS_Singapore   (2017-10-11)
Revision as of 14:18, 12 October 2017 by David135 (Talk | contribs)


Phosphate-dependent, temperature-sensitive cascaded system with IM2 expression

This design of this cascade system is similar to our other part BBa_K2447015, with the only exception where the GFP coding sequence is being replaced by E2 immunity protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1026
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2085
    Illegal XhoI site found at 1311
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1614
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/probiotics
//collections/probiotics/biocontainment
Parameters
None